BS James Madison University
PhD University of Wisconsin-Madison
Dr. Craig is interested in studying a short protein molecule called LL37which has been implicated in the onset of certain autoimmune diseases. LL37 can bind to DNA and fold DNA into small packages, thus allowing DNA to enter cells by facilitating their movement across the cell membrane. A 2007 report by Lande et al. in Nature suggests that LL37-mediated uptake of DNA by certain immune cells may incite an immune response to self-DNA, leading to the production of anti-DNA antibodies that are characteristic of autoimmune diseases such as systemic lupus erythematosus (SLE) and psoriasis.
Although much is known about the structure and function of LL37, the details of the binding interaction between LL37 and DNA remain to be elucidated. How does LL37 fold DNA into small packages and allow DNA to cross the cell membrane? Does LL37 recognize specific DNA sequences? How many LL37 molecules bind to DNA at one time? Dr. Craig proposes to investigate these questions by performing specific biophysical chemistry studies that are well-suited to undergraduate research. For instance, a decrease in the strength of binding of LL37 to DNA as the salt concentration is increased indicates a lack of sequence-specificity.
BA Hampshire College
PhD Oregon State University
Postdoctoral Duke University
Dr. Ruiz-Haas’ research interests are focused around the areas of analytical and environmental chemistry, with a focus on the chemical analysis of environmental samples, with an emphasis on analysis of hormones and endocrine disrupting compounds in the Shenandoah River, which is the main tributary to the Potomac River. Dr. Ruiz-Haas was recently awarded a grant from NIH to fund this research for the next two years
In addition, his research is centered interested in the development of analytical techniques and devices for low cost and/or field testing, as well as monitoring of redox transformations of organic pollutants in the environment by microorganisms. His students are also using UV light and ozone to destroy or remove traces of pharmaceuticals and personal care products in drinking and waste waters.